کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2467065 | 1555384 | 2012 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Impact of a mutator phenotype on motility and cell adherence in Salmonella Heidelberg Impact of a mutator phenotype on motility and cell adherence in Salmonella Heidelberg](/preview/png/2467065.png)
In this study, we investigated adherence and motility of the hypermutator Salmonella enterica Heidelberg B182 bovine strain related to a 12 bp deletion in mutS. This mutator phenotype was associated with increased adherence to epithelial cells and with high expression of fimA as shown by real-time RT-PCR. Motility studies showed that fliC were up-regulated in the B182 strain, while fljA and fljB were down-regulated.In order to determine if mutated mutS is implicated in this genes expression, isogenic strains, derived from a WT strain, containing the 12 bp deletion in mutS (Δ12bpmutS) or an inactivated mutS (ΔmutS) were generated. Δ12bpmutS and ΔmutS strains showed a spontaneous mutation rate similar to the environmental strain B182, but exhibited lower adherence capacity and fimA expression. In contrast to the fimbriae genes, in Δ12bpmutS, fliC expression was up-regulated, but fljA and fljB expression were decreased, as in the B182 strain. Only fljB expression was increased in ΔmutS mutants.Taken together, our data suggest that mutS alteration does not influence fimbriae expression but can impact flagella genes.
Journal: Veterinary Microbiology - Volume 159, Issues 1–2, 14 September 2012, Pages 99–106