کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2467304 | 1555390 | 2012 | 5 صفحه PDF | دانلود رایگان |
The amino acid sequence (TAVSPTTLR, 829–837 aa) on the glycoprotein E2 of classical swine fever virus (CSFV) is a conserved and linear neutralizing epitope. In the present study, two peptides were constructed based the core sequence of this neutralizing epitope, the dendrimeric peptide (Th-B4) containing four copies of B cell epitope fused to one copy of promiscuous T helper (Th) cell epitope and the peptide Th-B containing a single copy of B cell epitope fused to one copy of Th cell epitope. The dendrimeric peptide Th-B4 elicited high titers of neutralizing antibodies as detected in an indirect ELISA, blocking ELISA and neutralization test and induced a complete protection against CSFV C strain in rabbits. The Th-B elicited low titers of neutralizing antibodies and did not induce a protection in rabbits. These results suggest that the dendrimeric peptide Th-B4 may be a promising marker vaccine candidate against CSFV and the multimerization is a requirement for development of a peptide vaccine.
Journal: Veterinary Microbiology - Volume 156, Issues 1–2, 23 April 2012, Pages 200–204