کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2467758 1111992 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis
چکیده انگلیسی

Haemophilus parasuis causes contagious porcine Glässer's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of H. parasuis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 148, Issue 1, 24 February 2011, Pages 89–92
نویسندگان
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