کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2467999 | 1555417 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Detection of bluetongue serotype 4 in mouflons (Ovis aries musimon) from Spain Detection of bluetongue serotype 4 in mouflons (Ovis aries musimon) from Spain](/preview/png/2467999.png)
Bluetongue serotype 4 (BTV4) has been detected for the first time in tissue samples from 2 mouflons (Ovis aries musimon) from the South of Spain, in a retrospective study. The samples included in this study had been fixed and paraffin-embedded for over a year prior to their analysis using a BTV group-specific and a BTV4-specific RT-PCR test. Lung and lymphatic nodes were found positive in both specimens. The amplified DNA was confirmed to be BTV4 by sequencing the RT-PCR products and comparing them with other sequences from GenBank. The combination of RNA extraction from paraffin-embedded samples and serotype-specific real-time RT-PCR assays provides the tools for the detection of BTV from samples stored for a long time. The results shown in this study set out the basis for a greater survey with fixed samples from different species of wild ruminants that the veterinary services have been collecting for years.
Journal: Veterinary Microbiology - Volume 141, Issues 1–2, 24 February 2010, Pages 164–167