کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2468231 1555421 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of triple reassortant H1N1 influenza A viruses from swine in Ohio
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Characterization of triple reassortant H1N1 influenza A viruses from swine in Ohio
چکیده انگلیسی

An H1N1 influenza A virus, A/swine/Ohio/24366/07, was isolated from pigs in an Ohio county fair. Twenty-six people who came in contact with the infected pigs developed respiratory disease and two of these people were laboratory confirmed as H1N1 by the Centers for Disease Control and Prevention (CDC). The A/swine/Ohio/24366/07 virus we isolated from swine was shown at the CDC to have 100% identical genome sequence to the human virus associated with the county fair. This prompted us to characterize three swine and two human origin H1N1 influenza A viruses isolated at different time points in the State of Ohio. The three swine viruses were shown to be triple reassortant viruses harboring genes of human (PB1), swine (HA, NA, NP, M, and NS), and avian (PB2 and PA) lineage viruses. Although viruses evaluated in this study were isolated during a short time interval (3 years), genetic drift was observed within the HA and NA genes, including changes at the receptor binding and antigenic sites of HA1 protein. Nevertheless, all viruses exhibited antigenic similarity as evaluated with hemagglutination inhibition and virus neutralizing tests. Internal genes were similar to other reassortant viruses of various subtypes currently circulating in the United States. Interestingly, two of the swine viruses including the 2007 isolate replicated well in human airway epithelial cells, however, another virus isolated in 2006 showed very little replication.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 139, Issues 1–2, 20 October 2009, Pages 132–139
نویسندگان
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