Variation in Fusobacterium necrophorum strains present on the hooves of footrot infected sheep, goats and cattle

Footrot is a disease of sheep, goats and cattle that causes losses in production and raises welfare issues world-wide. The disease is characterised by destruction of the hard keratin of the hoof leading to lameness, and both Dichelobacter nodosus (D. nodosus) and Fusobacterium necrophorum (F. necrophorum) are thought to be involved in the etiology of this disease. While a lot is known about the genetic diversity of D. nodosus, very little is known about variation in F. necrophorum, especially as regards its role in footrot. We used PCR in conjunction with SSCP and sequencing to analyse swabs collected from the hooves of sheep, goats and cattle with symptomatic footrot for the presence of a portion of the lktA gene of F. necrophorum. Out of 29 samples tested, 27 had amplifiable lktA sequences and within these we found four different variants of the lktA gene. Eight of the nine samples from cattle were positive for a variant that matched the type strain of F. necrophorum subsp. necrophorum. Of the 14 samples from sheep, 13 were positive for lktA, but none of theses matched the known type strains, and 11/13 of the lktA sequences were identical. This sequence was distinct to those of the type strains. None of the footrot infections carried multiple variants of lktA, suggesting that only one strain of F. necrophorum is present in each case. This is in contrast to D. nodosus in footrot infections, which have been demonstrated to have up to seven strains infecting a single hoof.