کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2471903 1555776 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fecal and serological survey of Neospora caninum in farm dogs in Costa Rica
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Fecal and serological survey of Neospora caninum in farm dogs in Costa Rica
چکیده انگلیسی

To detect oocysts of Neospora caninum in dog feces and to determine the excretion pattern in dogs from specialized dairy farms in Costa Rica, a total of 265 fecal samples from 34 dogs were collected at intervals from February to August 2005. Fecal samples were examined for N. caninum-like oocysts microscopically, by DNA detection using the polymerase chain reaction (PCR), and by bioassay. N. caninum DNA was detected by PCR in four fecal samples, twice from one dog, but oocysts were not detected microscopically in these dogs. Sera of 31 of 34 dogs were tested for antibodies to N. caninum by a competitive-inhibition ELISA (VMRD®). Fifteen (48.4%) of 31 dogs had antibodies to N. caninum by ELISA. Seroconversion was not found in 28 dogs that were bled twice, 4 months apart (March and July 2005). Only one dog tested positive to N. caninum by both ELISA and PCR. This is the first report of finding N. caninum DNA in feces of naturally infected dogs in Costa Rican dairy farms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 149, Issues 3–4, 10 November 2007, Pages 265–270
نویسندگان
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