کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2484307 | 1114305 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative Targeted Absolute Proteomics for 28 Transporters in Brush-Border and Basolateral Membrane Fractions of Rat Kidney
ترجمه فارسی عنوان
پروتئومیکس مطلق هدفمند برای 28 محرک در بخش های غشایی برش و مرزی بر روی کلیه موش صحرایی
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کلمات کلیدی
SRM/MRMBBMABCSLCBLMHPLC (high-performance/pressure liquid chromatography) - HPLC (کروماتوگرافی مایع با کارایی بالا / فشار)LC-MS/MS - LC-MS / MSsolute transporters - انتقال دهنده هاsolute carrier - حامل رقیبMembrane transport - حمل غشاءDrug transport - حمل مواد مخدرABC transporters - حمل کننده ABCMembrane transporter - حمل کننده غشاییRenal excretion - دفع کلیویBBB - سد خونی مغزیMass spectrometry - طیف سنجی جرمیLiquid chromatography-tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع دو طرفهBrush-border membrane - غشای مرز برسBasolateral membrane - غشای نازکBlood-brain barrier - مانع خون مغزیProteomics - پروتئومیکسProteins - پروتئین هاATP-binding cassette - کیت اتصال به ATP
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
چکیده انگلیسی
The purpose of the present study was to determine the absolute protein expression levels of various transporters in renal brush-border membrane (BBM) and basolateral membrane (BLM) fractions, in order to understand the quantitative differences in average transport activities among different transporters at each cellular membrane. BBM and BLM fractions of rat kidney were prepared and digested with trypsin, and simultaneous absolute quantification of 28 transporters and a BLM marker, Na+/K+-ATPase, was performed using our established quantitative-targeted absolute proteomics (QTAP) technique. In BBM fraction, the protein expression levels of bcrp, urat1, mate1, octl1, mrp4, mdr1a, and abca3 were 40.3, 22.2, 8.90, 4.85, 4.69, 3.22, and 0.976 fmol/μg protein, respectively. In BLM fraction, the protein expression levels of oat1, oat3, oct1, mrp6, and mrp1 were 10.6, 10.2, 4.59, 0.724, and 0.271 fmol/μg protein, respectively. The expression levels of abca2, abca4, abca5, abca12, abcb4, mrp5, abcc9, abcg1, abcg5, lat1, ntcp, pgt, oatp2b1, oatp1b2, oatp3a1, and oct3 were under the limit of quantification in both fractions. The quantitative transporter protein expression profiles at these membranes, as determined by QTAP analysis, should be helpful to understand the contributions of individual transporters to renal excretion of xenobiotics and endogenous compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 105, Issue 2, February 2016, Pages 1011-1016
Journal: Journal of Pharmaceutical Sciences - Volume 105, Issue 2, February 2016, Pages 1011-1016
نویسندگان
Yasuo Uchida, Takafumi Toyohara, Sumio Ohtsuki, Yoshinori Moriyama, Takaaki Abe, Tetsuya Terasaki,