کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2484664 | 1114322 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Formulation and In Vitro Characterization of Thermosensitive Liposomes for the Delivery of Irinotecan
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
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چکیده انگلیسی
The effect of the lipid composition on the physicochemical properties of a liposomal carrier containing irinotecan (CPT-11) and its in vitro antitumoral activity on a colon cancer cell line has been investigated. The paper describes the procurement of a novel and easy-to-prepare temperature-sensitive carrier for CPT-11 and proves its suitability as delivery system on the basis of its ability to incorporate the drug and on its efficiency to promote drug internalization. Permeability data, studied in vitro in a simulated biological medium at different temperatures, showed that both the nature of the lipids and the ratio in which they have been mixed play a key role in the release of the encapsulated product. Stable temperature-sensitive liposomes with good drug incorporation efficiency were obtained and characterized. The cellular uptake and the cytotoxic activity of the CPT-11 liposomal formulation were compared with those corresponding to the free drug with promising results, being concentration and time dependent and significantly higher. Thus, it could be interpreted that the greatest cytotoxic effect of liposomal CPT-11 was because of the increased uptake provided by the liposomal carrier. Studies of cell cycle and annexin V binding showed drug-induced changes in cell cycle dynamics and the proapoptotic effect of liposomal CPT-11. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:3127-3138, 2014
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 10, October 2014, Pages 3127-3138
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 10, October 2014, Pages 3127-3138
نویسندگان
Ana Casadó, Maria Lluïsa Sagristá, Margarita Mora,