کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2485129 | 1114345 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Paracellular Porosity and Pore Size of the Human Intestinal Epithelium in Tissue and Cell Culture Models
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Paracellular Porosity and Pore Size of the Human Intestinal Epithelium in Tissue and Cell Culture Models Paracellular Porosity and Pore Size of the Human Intestinal Epithelium in Tissue and Cell Culture Models](/preview/png/2485129.png)
چکیده انگلیسی
The paracellular space defines the passive permeation of hydrophilic compounds in epithelia. The goal of this study was to characterise the paracellular permeation pathway in the human intestinal wall and differentiated epithelial cell models (MDCKII, Cacoâ2 and 2/4/A1). The permeabilities of hydrophilic polyethylene glycols (PEG) were investigated in diffusion chambers, and mass spectrometry was used to obtain accurate concentrations for each PEG molecule. The paracellular porosity and the size of the pores in the membranes were estimated from the PEG permeability data using an effusionâbased approach. The porosities were found to be low (fraction 10â7-10â5 of the epithelial surface) in all investigated membranes. Two different pore sizes (radii 5-6 and >10âÃ
) were detected in the human intestinal epithelium and the Cacoâ2 and MDCKII cells, while only one (about 15âÃ
) in the 2/4/A1 monolayer. The paracellular porosities of the human small intestine and 2/4/A1 monolayers were larger (>10â7) than that of the MDCKII and Cacoâ2 cells (<10â7). We report for the first time the quantitative values describing both porosity and pore size of the paracellular space in the human intestine. The cell models deviate from the small intestine either with respect to porosity (Cacoâ2, MDCKII) or pore size distribution (2/4/A1). © 2009 WileyâLiss, Inc. and the American Pharmacists Association J Pharm Sci 99: 2166-2175, 2010
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 99, Issue 4, April 2010, Pages 2166-2175
Journal: Journal of Pharmaceutical Sciences - Volume 99, Issue 4, April 2010, Pages 2166-2175
نویسندگان
Johanna Linnankoski, Johanna Mäkelä, Joni Palmgren, Timo Mauriala, Charlotta Vedin, AnnaâLena Ungell, Lucia Lazorova, Per Artursson, Arto Urtti, Marjo Yliperttula,