کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2486059 | 1114374 | 2010 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Specific interactions in high concentration antibody solutions resulting in high viscosity
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Specific interactions in high concentration antibody solutions resulting in high viscosity Specific interactions in high concentration antibody solutions resulting in high viscosity](/preview/png/2486059.png)
چکیده انگلیسی
The purpose of this work was to investigate the selfâassociating behavior observed in high concentration monoclonal antibody (MAb1) solutions at pH 6.0. Zeta potential measurements over the pH range (4.0-9.0) showed lower net charges present on the molecule at pH 6.0 and 7.0. The point of zero charge or crossover from positive to negative potential was at pH 6.7 and was different from the theoretical isoelectric point (pI) of 7.8. Interaction parameter (kD) from dynamic light scattering (DLS) studies indicated that attractive interactions of a similar magnitude span over a pH range of 5.0-8.0. Change in kD values with increase in ionic strength corroborated that the interactions were electrostatic in origin. Comparative rheology analysis of three different monoclonal antibodies at pH 6.0, using ultrasonic shear rheometer, showed a high solution storage modulus (Gâ²) and its sharp increase in magnitude at high concentrations in MAb1. The shape of the rheology profile was indicative of strong attractive interactions between solute molecules. High specific attractive interactions may be attributed to charge-charge and charge-dipole interactions at the surface in the Fab regions of MAb1, and gives a reasonable explanation for the pH dependency of the rheological profile observed. © 2009 WileyâLiss, Inc. and the American Pharmacists Association J Pharm Sci 99: 1152-1168, 2010
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 99, Issue 3, March 2010, Pages 1152-1168
Journal: Journal of Pharmaceutical Sciences - Volume 99, Issue 3, March 2010, Pages 1152-1168
نویسندگان
Sandeep Yadav, Jun Liu, Steven J. Shire, Devendra S. Kalonia,