کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2487280 | 1114410 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of Nitroxyl Spin Probes in Mouse Brain by X-Band ESR with Microdialysis Technique
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کلمات کلیدی
ROS, reactive oxygen species.BBB, blood–brain barrier - BBB، مانع خونریزی مغزیESR, electron spin resonance - ESR، رزونانس اسپین الکترونNitroxyl radical - رادیکال نیتروکسیلFree radical - رادیکالهای آزاد Electron spin resonance - رزونانس اسپین الکترونBlood–brain barrier - سد خونی مغزیPharmacokinetics - فارماکوکینتیکmicrodialysis - میکرو دیالیزMW, molecular weight - وزن مولکولی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
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چکیده انگلیسی
Stable nitroxyl radicals are widely used in electron spin resonance (ESR) studies in vivo to determine ROS generation, but there are insufficient data on how their distribution to various tissues, excretion, and/or systemic signal decay affect the signal decay in a region of interest. Here, we evaluated the level of spin probe in the brain using a microdialysis combined with X-band ESR spectroscopy, to clarify the BBB permeability of different spin probes. We also determined the association between PROXYL spin probe signal decay in the head and the probe's level in the brain, its excretion in urine, and its rate of signal decay in other areas and tissues. Dialysate recovered from the mouse prefrontal cortex was used to determine the total spin probe level in the brain by X-band ESR spectroscopy. There was a positive correlation between the level of spin probes in the brain and their partition coefficients. Furthermore, the in vivo decay rate of the nitroxyl radical signal in the head was associated with the probes' level in the brain, but not with its systemic signal decay rate or excretion into urine. These basic data may support the use of PROXYLs as site-specific ROS probes in the brain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 97, Issue 9, September 2008, Pages 4101-4107
Journal: Journal of Pharmaceutical Sciences - Volume 97, Issue 9, September 2008, Pages 4101-4107
نویسندگان
Takeshi Shiba, Mayumi Yamato, Wataru Kudou, Kazuhiro Ichikawa, Ken-Ichi Yamada, Toshiaki Watanabe, Hideo Utsumi,