کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2487728 | 1114429 | 2007 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stabilization of a hydrophobic recombinant cytokine by human serum albumin
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The objective was to evaluate the impact of pH and NaCl content on aggregation, particle formation, and solubility of a hydrophobic recombinant human cytokine in formulations with human serum albumin (HSA) as stabilizing excipient. While cytokineâHSA formulations were stable at physiological pH, a tremendous increase in turbidity at pH 5.0, close to the isoelectric point of HSA was caused by a partially irreversible precipitation. By dynamic light scattering (DLS), disc centrifugation, atomic force microscopy (AFM), and light obscuration it could be shown that the turbidity was mainly caused by particles larger than 120 nm. SDS-PAGE provided evidence that the precipitation at pH 5.0 was mainly caused by the cytokine. The HSAâstabilizers Naâoctanoate and NaâNâacetyltryptophante were less effective in preventing the turbidity increase of unstabilizedâHSA compared to NaCl. The interactions between HSA and cytokine were weakened by NaCl, as determined by fluorescence spectroscopy. The positive effect of NaCl on the formulation could be attributed to a direct stabilization of HSA and weaker interactions between HSA and the cytokine, which in consequence provided an overall stabilization of the cytokine. © 2007 WileyâLiss, Inc. and the American Pharmacists Association J Pharm Sci 96: 2987-2999, 2007
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 96, Issue 11, November 2007, Pages 2987-2999
Journal: Journal of Pharmaceutical Sciences - Volume 96, Issue 11, November 2007, Pages 2987-2999
نویسندگان
Andrea Hawe, Wolfgang Friess,