کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2487780 | 1114431 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Solid Phase Extraction as a Faster Alternative to HPLC: Application to MS Analysis of Metabolic Stability Samples
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Due to recent advances in high throughput organic synthesis, discovery teams now need to profile increased numbers of analogs in vitro for their absorption, distribution, metabolism, and excretion (ADME) properties. Consequently, pharmaceutical companies are developing lower cost and higher throughput methods for ADME testing. As demands for metabolic stability testing have increased in our laboratory, the time required to analyze samples using highâpressure liquid chromatographyâmass spectrometry (HPLCâMS) has grown rapidly and ultimately limited our data output. In this study we show that solid phase extractionâmass spectrometry (SPEâMS) is a viable alternative to HPLCâMS for monitoring small molecule stability in liver microsomes. Using the SPEâMS approach, samples can be analyzed in 24Â s compared to 2.5Â min on the HPLCâMS without compromising data quality, thereby alleviating the analytical bottleneck.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 96, Issue 6, June 2007, Pages 1619-1624
Journal: Journal of Pharmaceutical Sciences - Volume 96, Issue 6, June 2007, Pages 1619-1624
نویسندگان
Brittany L. Inman, Linda E. Chovan, Peter J. Dandliker, Yau Yi Lau,