کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
25014 43552 2007 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolic flux analysis using stoichiometric models for Aspergillus niger: Comparison under glucoamylase-producing and non-producing conditions
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Metabolic flux analysis using stoichiometric models for Aspergillus niger: Comparison under glucoamylase-producing and non-producing conditions
چکیده انگلیسی

Aspergillus niger AB1.13 cultures with glucoamylase production (with d-glucose as substrate) and without glucoamylase production (with d-xylose as substrate) were characterized by metabolic flux analysis. Two comprehensive metabolic models for d-glucose- as well as for d-xylose-consumption were used to quantify and compare the metabolic fluxes through the central pathways of carbon metabolism at different pH-values. The models consist of the most relevant metabolic pathways for A. niger including glycolysis, pentose-phosphate pathway, citrate cycle, energy metabolism and anaplerotic reactions comprising two intracellular compartments, the cytoplasm and mitochondrion.When d-xylose was used as the sole carbon source, the relative flux of the substrate through the oxidative pentose-phosphate pathway (PPP) via G6P-dehydrogenase was unaffected by the pH-value of the culture medium. About 30% of d-xylose consumed was routed through the oxidative PPP. In contrast, the flux of d-glucose (i.e., under glucoamylase-producing conditions) through the oxidative PPP was remarkably higher and, in addition was significantly affected by the pH-value of the culture medium (40% at pH 5.5, 56% at pH 3.7, respectively). Summarizing, the flux through the PPP under glucoamylase producing conditions was 30–90% higher than for non-producing conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 132, Issue 4, 1 December 2007, Pages 405–417
نویسندگان
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