کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2502864 1557405 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The in vivo antitumor activity of LHRH targeted methotrexate–human serum albumin nanoparticles in 4T1 tumor-bearing Balb/c mice
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
پیش نمایش صفحه اول مقاله
The in vivo antitumor activity of LHRH targeted methotrexate–human serum albumin nanoparticles in 4T1 tumor-bearing Balb/c mice
چکیده انگلیسی

The use of targeted drug delivery systems is a growing trend in cancer treatment to decrease the adverse effect of anti-cancer drugs. In this study, we sought to conjugate methotrexate–human serum albumin nanoparticles (MTX–HSA NPs) with luteinizing-hormone releasing hormone (LHRH). The LHRH was intended to target LHRH receptors overexpressed on the several types of tumors. The expression of LHRH receptors on the 4T1 breast cancer cells was confirmed by FITC conjugated LHRH receptor antibody using fluorescence microscopy. Female Balb/c mice bearing 4T1 breast cancer tumor were treated with a single i.v. injection of free MTX, non-targeted MTX–HSA NPs and LHRH targeted MTX–HSA NPs. LHRH targeted MTX–HSA nanoparticles showed stronger anti-tumor activity in vivo. By 7 days after treatment, average tumor volume in the LHRH targeted MTX–HSA NPs treated group decreased to 8.67% of the initial tumor volume when the number of attached LHRH molecules on MTX–HSA NPs was the highest, while the average tumor volume in non-targeted MTX–HSA NPs treated mice grew rapidly and reached 250.7% of the initial tumor volume 7 days after the treatment. LHRH targeted MTX–HSA NPs could significantly extend the survival time of tumor bearing mice compared with the non-targeted MTX–HSA NPs and free MTX formulations.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 431, Issues 1–2, 15 July 2012, Pages 183–189
نویسندگان
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