کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2503646 | 1557437 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Equilibrium drug solubility measurements in 96-well plates reveal similar drug solubilities in phosphate buffer pH 6.8 and human intestinal fluid Equilibrium drug solubility measurements in 96-well plates reveal similar drug solubilities in phosphate buffer pH 6.8 and human intestinal fluid](/preview/png/2503646.png)
This study was conducted to develop a high throughput screening (HTS) method for the assessment of equilibrium solubility of drugs. Solid-state compounds were precipitated from methanol in 96-well plates, in order to eliminate the effect of co-solvent. Solubility of twenty model drugs was analyzed in water and aqueous solutions (pH 1.2 and 6.8) in 96-well plates and in shake-flasks (UV detection). The results obtained with the 96-well plate method correlated well (R2 = 0.93) between the shake-flask and 96-well plates over the wide concentration scale of 0.002–169.2 mg/ml. Thereafter, the solubility tests in 96-well plates were performed using fasted state human intestinal fluid (HIF) from duodenum of healthy volunteers. The values of solubility were similar in phosphate buffer solution (pH 6.8) and HIF over the solubility range of 102–105 μg/ml. The new 96-well plate method is useful for the screening of equilibrium drug solubility during the drug discovery process and it also allows the use of human intestinal fluid in solubility screening.
The 96-well plate equilibrium drug solubility results in human intestinal fluid and aqueous buffer at pH 6.8.Figure optionsDownload as PowerPoint slide
Journal: International Journal of Pharmaceutics - Volume 405, Issues 1–2, 28 February 2011, Pages 132–136