کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2504195 | 1557453 | 2010 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Junk DNA enhances pEI-based non-viral gene delivery Junk DNA enhances pEI-based non-viral gene delivery](/preview/png/2504195.png)
Gene therapy aims at delivering exogenous DNA into the nuclei of target cells to establish expression of a therapeutic protein. Non-viral gene delivery is examined as a safer alternative to viral approaches, but is presently characterized by a low efficiency. In the past years several non-viral delivery strategies have been developed, including cationic polymer-based delivery. One of the most described and most active polymers is linear pEI. This study addresses questions regarding formulating highly efficient pEI-based polyplexes. By mixing reporter plasmid DNA with non-coding junk DNA it was shown that the amount of reporter plasmid can be significantly decreased in linear pEI-based transfection while maintaining transfer activity. Junk DNA maximally exerts its function when co-delivered with active DNA within the same pEI complexes rather than upon co-delivery of distinct junk DNA/pEI and active DNA/pEI complexes. We conclude that not the total amount of active DNA, but rather the total amount of active DNA-containing particles is the limiting factor in pEI-mediated transfection.
Journal: International Journal of Pharmaceutics - Volume 390, Issue 1, 5 May 2010, Pages 76–83