کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2507832 | 1117511 | 2014 | 6 صفحه PDF | دانلود رایگان |
A simple, eco-friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β-hydroxyethyl)rutosides fluorescence enhancement, increasing considerably the sensitivity of detection. All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL/min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for O-(β-hydroxyethyl)rutosides in the range of 0.01–200 µg/mL with a detection limit of 0.001 µg/mL (s/n=3). Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of O-(β-hydroxyethyl)rutosides in bulks as well as in commercial formulations.
Journal: Journal of Pharmaceutical Analysis - Volume 4, Issue 6, December 2014, Pages 368–373