کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2510064 1117949 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nucleic acid-induced antiviral immunity in shrimp
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Nucleic acid-induced antiviral immunity in shrimp
چکیده انگلیسی


• The injection of nucleic acid mimics afforded shrimp antiviral immunity.
• Genes involved in poly(I:C)-induced shrimp antiviral immunity were identified using the SSH method.
• L. vannamei IKKε could significantly activate IFN reporter genes in HEK293T cells.
• In crustacean databases, many genes homologous to genes of the vertebrate IFN response were discovered.

Vertebrates detect viral infection predominantly by sensing viral nucleic acids to produce type I interferon (IFN). In invertebrates, it has been believed that the IFN system is absent and RNA interference is a sequence-specific antiviral pathway. In this study, we found that injection of nucleic acid mimics poly(I:C), poly(C:G), CL097, poly C and CpG-DNA, afforded shrimp antiviral immunity, which is similar to the vertebrate IFN system. Using suppression subtractive hybridization (SSH) method, 480 expression sequence tags were identified to be involved in the poly(I:C)-induced antiviral immunity of the model crustacean Litopenaeus vannamei, and 41% of them were new genes. In the SSH libraries, several IFN system-related genes such as dsRNA-dependent protein kinase PKR, Toll-like receptor 3 (TLR3) and IFNγ-inducible protein 30 were identified. L. vannamei IKKε, whose vertebrate homologs are central regulators of the IFN-producing pathway, could significantly activate IFN reporter genes in HEK293T cells. In crustacean databases, many genes homologous to genes of the vertebrate IFN response, such as IRFs, PKR, ADAR (adenosine deaminase, RNA-specific) and other interferon-stimulated genes (ISGs) were discovered. These results suggest that shrimp may possess nucleic acid-induced antiviral immunity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Antiviral Research - Volume 99, Issue 3, September 2013, Pages 270–280
نویسندگان
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