کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2510150 1117954 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro inhibition of influenza A virus infection by marine microalga-derived sulfated polysaccharide p-KG03
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
In vitro inhibition of influenza A virus infection by marine microalga-derived sulfated polysaccharide p-KG03
چکیده انگلیسی

The sulfated polysaccharide, p-KG03, purified from the marine microalga, Gyrodinium impudium, is a unique compound comprising homogenous galactose units conjugated to uronic acid and sulfated groups. Although previous studies showed that p-KG03 suppresses tumor cell growth and infection by encephalomyocarditis virus, its effect against enveloped virus infection and the biological mechanism of action have not been elucidated. In this report, the inhibitory activity of p-KG03 against influenza virus was examined and compared with that of other sulfated polysaccharides (fucoidan and pentosan polysulfate) and antiviral agents (oseltamivir phosphate, oseltamivir carboxylate, amantadine, and ribavirin). The results of a cytopathic effect reduction assay using MDCK cells demonstrated that p-KG03 exhibited the 50% effective concentration (EC50) values of 0.19–0.48 μg/ml against influenza type A virus infection (selectivity index >200) but not all influenza type B viruses. Mechanism studies showed that inhibition of influenza virus replication was maximized when p-KG03 was added during or within 6 h after viral infection, suggesting that mainly the viral adsorption and internalization steps are targeted by this compound. The results of influenza virus binding assay to p-KG03 and fluorescence microscopy indicate that the antiviral activity of p-KG03 is directly associated with its interaction with viral particles. The sulfated polysaccharide p-KG03 is a potent and specific influenza A viral entry inhibitor and may be a candidate for antiviral drug development.


► The sulfated polysaccharide p-KG03 is derived from red marine microalga.
► It is active against influenza A virus but not all influenza B virus in vitro.
► The antiviral activity is highly correlated with its affinity to viral particles.
► The main mode of action of p-KG03 is to interfere with viral entry into cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Antiviral Research - Volume 93, Issue 2, February 2012, Pages 253–259
نویسندگان
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