Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in Brassica pekinensis plants

► We study antiviral effect of tenofovir on CaMV in Brassica pekinensis grown in vitro. ► Tenofovir (25-50 mg/l) reduced CaMV concentration in plants within 6-9 weeks. ► Virus-free plants were obtained after 3-month cultivation on medium with tenofovir. ► We proved metabolism of tenofovir in B. pekinensis leaves but not in roots. ► Mono- and diphosphate analogs of NDP and/or NTP are the only metabolites formed.