کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2510656 | 1117978 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Improved protection against avian influenza H5N1 virus by a single vaccination with virus-like particles in skin using microneedles Improved protection against avian influenza H5N1 virus by a single vaccination with virus-like particles in skin using microneedles](/preview/png/2510656.png)
To develop a more effective vaccination method against H5N1 virus, we investigated the immunogenicity and protective efficacy after skin vaccination using microneedles coated with influenza virus-like particles containing hemagglutinin derived from A/Vietnam/1203/04 H5N1 virus (H5 VLPs). A single microneedle vaccination of mice with H5 VLPs induced increased levels of antibodies and provided complete protection against lethal challenge without apparent disease symptoms. In contrast, intramuscular injection with the same vaccine dose showed low levels of antibodies and provided only partial protection accompanied by severe body weight loss. Post-challenge analysis suggested that improved protection was associated with lower lung viral titers and enhanced generation of recall antibody secreting cells by microneedle vaccination. Thus, this study provides evidence that skin delivery of H5 VLP vaccines using microneedles designed for self-administration induces improved protection compared to conventional intramuscular immunization.
Journal: Antiviral Research - Volume 88, Issue 2, November 2010, Pages 244–247