کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
25219 | 43561 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bacterial protein patterning by micro-contact printing of PLL-g-PEG
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Bacterial protein patterning by micro-contact printing of PLL-g-PEG Bacterial protein patterning by micro-contact printing of PLL-g-PEG](/preview/png/25219.png)
چکیده انگلیسی
Biomimetic micro-patterned surfaces of three S-layer (fusion) proteins, wild type (SbpA), enhanced green fluorescence protein (SbpA-EGFP) and streptavidin (SbpA-STV), were built by microcontact printing of poly-l-lysine grafted polyethylene glycol (PLL-g-PEG). The functionality of the adsorbed proteins was studied with atomic force microscopy and fluorescence microscopy. Atomic force microscopy (AFM) measurements showed that wild-type SbpA recrystallized on PLL-g-PEG free areas, while fluorescent properties of SbpA-EGFP and the interaction of SbpA-streptavidin heterotetramers with biotin were not affected due to the adsorption on the micro patterned substrates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 130, Issue 3, 30 June 2007, Pages 247–252
Journal: Journal of Biotechnology - Volume 130, Issue 3, 30 June 2007, Pages 247–252
نویسندگان
V. Saravia, S. Küpcü, M. Nolte, C. Huber, D. Pum, A. Fery, U.B. Sleytr, J.L. Toca-Herrera,