کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2524028 1557967 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Long noncoding RNA expression patterns in lymph node metastasis in colorectal cancer by microarray
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
Long noncoding RNA expression patterns in lymph node metastasis in colorectal cancer by microarray
چکیده انگلیسی

AimTo profile the long noncoding RNA (lncRNA) expression patterns in normal lymph node (NLN) of colorectal cancer compared with paired metastatic lymph node (MLN).Materials and methodsMLN, NLN lncRNA and messenger RNA (mRNA) expression levels were determined by microarray in three patients; quantitative reverse transcription–polymerase chain reaction validation of 3 differentially expressed lncRNAs was conducted in another 26 patients. The lncRNAs’ functions were predicted through coexpressed mRNA annotations.Result(s)A total of 1133 lncRNA transcripts transcripts were dysregulated in metastatic lymph node, compared with normal lymph node. The expressions of the 3 chosen lncRNAs were validated by quantitative reverse transcription–polymerase chain reaction. Functional analysis suggests that several groups of lncRNAs may participate in biological pathways related to colorectal cancer by cis- and/or trans-regulation of protein-coding genes.Conclusion(s)This study constitutes the first report of lncRNA expression patterns in human MLN, NLN of colorectal cancer. More than 1000 dysregulated lncRNA transcripts are found by microarray.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 75, October 2015, Pages 12–18
نویسندگان
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