کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2524309 | 1119555 | 2012 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 film deposited by cathodic arc Proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 film deposited by cathodic arc](/preview/png/2524309.png)
This study aimed at the proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 and pure TiO2 films prepared by cathodic arc deposition. The films were examined by X-ray photo-electron spectroscopy, which showed that silicon was successfully doped into the Si-TiO2 film. Meanwhile, no significant difference was found between the surface morphology of silicon-doped TiO2 and pure TiO2 films. When osteoblastic cells were cultured on silicon-doped TiO2 film, accelerated cell proliferation was observed. Furthermore, cell differentiation was evaluated using alkaline phosphatase (ALP), type I collagen (COL I) and osteocalcin (OC) as differentiation markers. It was found that ALP activity, the expression levels of OC gene, COL I gene and protein were up-regulated on silicon-doped TiO2 film at 3 and 5 days of culture. Moreover, no significant difference was found in apoptosis between the cells cultured on silicon-doped TiO2 and pure TiO2 films. Therefore, findings from this study indicate that silicon-doped film favors osteoblastic proliferation and differentiation, and has the potential for surface modification of implants in the future.
Journal: Biomedicine & Pharmacotherapy - Volume 66, Issue 8, December 2012, Pages 633–641