کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2524343 1119559 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chloride intracellular channel 1 is an important factor in the lymphatic metastasis of hepatocarcinoma
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
Chloride intracellular channel 1 is an important factor in the lymphatic metastasis of hepatocarcinoma
چکیده انگلیسی

We have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot (WB) analysis, respectively. The down-regulation of CLIC1 enhanced proliferative activity, increased the ratio of G2/M and decreased percentage of apoptosis. In addition, the capability of migration and invasion decreased significantly. The results indicate that CLIC1 is a critical factor in the development of lymphatic metastasis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 66, Issue 3, April 2012, Pages 167–172
نویسندگان
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