کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2524452 1119570 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The mechanism of the G0/G1 cell cycle phase arrest induced by activation of PXR in human cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
The mechanism of the G0/G1 cell cycle phase arrest induced by activation of PXR in human cells
چکیده انگلیسی

ContextPregnane X receptor (PXR) is an important transcriptional regulator that plays important roles in the cell metabolism and cell growth by regulating the transcriptional of a sort of metabolizing enzymes.ObjectiveTo investigate whether rifampicin effected HepG2 cells growth and the inhibition was due to the G0/G1 phase arrest.MethodsPXR-knockdown experiments using RNAi showed that the cell cycle phase arrest mediated by rifampicin based on activation of PXR. The results also indicated that cell phase arrest by rifampicin could protect cells form UVB-induced DNA damage. Retinoid X receptor alpha (RXRα) expression level in cells is another key factor for cell cycle phase arrest mediated by rifampicin. Both over expression and lacking expression of RXRα in cell reduced the cell arrest efficiency mediated by rifampicin. In the study, we found that rifampicin inhibited HepG2 cells growth and demonstrated that the inhibition is due to the G0/G1 phase arrest through flow cytometry analysis.ConclusionThe results showed that RXRα promote cell cycle phase transition rate of HepG2. Competitive bind of rifampicin-activated PXR with RXRα is one main reason to arrest cell cycle phase through inhibiting combination of RXRα with other partners. Rifampicin could promote cell growth rate when RXRα expressed more excessively than PXR in cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 65, Issue 7, October 2011, Pages 467–473
نویسندگان
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