Effect of aspirin on DNA damage induced by MMC in Drosophila

In our previous paper, we found that aspirin suppressed the genotoxicity of mitomycin C (MMC) in a somatic mutation and recombination test (SMART) in Drosophila melanogaster. In order to reveal the mechanism of antigenotoxicity of aspirin, we evaluated the protective effects of aspirin against the genotoxicity of MMC with the DNA repair test in Drosophila melanogaster. Three types of treatment of aspirin were performed as co-, post- and pre-treatment. Aspirin co-treatment suppressed effectively the genotoxicity of MMC in a dose-dependent manner and the sex ratio at a dose of aspirin 10 mg/bottle elevated from 0.01 (without aspirin) to 0.65 at sc z1 w+(TE) mei-9a mei-41D5/-C(1)DX, y f [mei-9 mei-41, Rec− male · Rec+ female] consists of DNA repair-deficient (Rec−) males and -proficient (Rec+) females. The antigenotoxic effect of aspirin on [mei-41, Rec− male · Rec+ female] was similar to that on [mei-9, Rec− male · Rec+ female]. But post- and pre-treatment by aspirin did not affect the genotoxicity of MMC on [mei-9 mei-41, Rec− male · Rec+ female].