کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2540627 | 1122601 | 2014 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Anti-inflammatory effects of 5-HT3 receptor antagonists in interleukin-1beta stimulated primary human chondrocytes Anti-inflammatory effects of 5-HT3 receptor antagonists in interleukin-1beta stimulated primary human chondrocytes](/preview/png/2540627.png)
• The 5-HT3R antagonist dolasetron inhibits IL-1β-induced IL-6 release in chondrocytes.
• Dolasetron and tropisetron prevent IL-1β-induced PGE2 synthesis in chondrocytes.
• Dolasetron does not affect IL-1β-induced COX-2 and mPGES-1 expression.
• Effects of dolasetron on COX-2-mediated PGE2 production may be mediated through its interaction with the peroxidation site of COX-2.
BackgroundChondrocytes are one of the main cell types involved in rheumatoid inflammation, releasing mediators which add to cartilage destruction, bone damage and consequently disability. Current evidence suggests that serotonin 5-HT3 receptor antagonists (5-HT3RA) show anti-inflammatory and antioxidant properties in vitro and in vivo. Yet, the mechanisms of the anti-inflammatory effects of 5-HT3RA have not been elucidated in detail.MethodsTherefore, we examined in detail the effects of 5-HT3RA on inflammatory parameters in human primary chondrocytes in vitro by studying prostaglandin E2 (PGE2) and 8-isoprostane (8-iso-PGF2α) levels by EIA and interleukin-6 (IL-6) synthesis by ELISA. Cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) protein levels were analyzed by Western blot.ResultsWe found a significant reduction of IL-1β induced PGE2, 8-iso-PGF2β and IL-6 chondrocytes by 5-HT3RA especially by dolasetron.ConclusionsThis study provides additional support to the potential use of 5-HT3RAs as therapeutic agents to reduce joint inflammation.
Journal: International Immunopharmacology - Volume 22, Issue 1, September 2014, Pages 160–166