کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
25411 43572 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
چکیده انگلیسی

Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l−1. The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 124, Issue 2, 13 July 2006, Pages 469–472
نویسندگان
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