کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2541698 | 1122671 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Histamine affects STAT6 phosphorylation via its effects on IL-4 secretion: Role of H1 receptors in the regulation of IL-4 production
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کلمات کلیدی
IL-4H1 receptorspKainositol 1, 4, 5-trisphosphateTh2H4 receptorIP3Th1PLCStat6PKCJanus kinase - کیناز جانوس PMA - LDC هاSTAT - آمارphospholipase C - فسفولیپاز CSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیHistamine - هیستامینprotein kinase A - پروتئین کیناز AProtein kinase C - پروتئین کیناز سیJAK - چگونهH1 receptor - گیرنده H1H2 receptor - گیرنده H2H3 receptor - گیرنده H3histamine receptor 1 - گیرنده هیستامین 1Histamine receptor 2 - گیرنده هیستامین 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Histamine alters the Th1/Th2 cytokine balance towards the Th2 cytokine profile and consequently plays a role in allergic diseases and asthma. This study was designed to investigate the effects of histamine on the STAT6 phosphorylation. C57/BL6 splenocytes were pretreated with different concentrations of histamine (10â 4 M to 10â 13 M) followed by stimulation with PMA+ionomycin or IL-4. The phosphorylated and total basal STAT6 levels were assessed by employing the immunoblotting technique. Histamine caused the hyper-phosphorylation of STAT6. H1 receptor antagonist pyrilamine reversed the effect of histamine on STAT6 phosphorylation. However, H2 receptor antagonist ranitidine and H3/H4 receptor antagonist thioperamide did not affect the histamine mediated hyper-phosphorylation of STAT6. Furthermore, H1 receptor agonist betahistine enhanced the phosphorylation of STAT6 whereas H2 receptor agonist amthamine did not affect the phosphorylation STAT6. Furthermore, tyrosine kinase inhibitor, tyrphostin, inhibited the histamine mediated phosphorylation of STAT6 when stimulated with PMA + ionomycin. The effects of histamine on the STAT6 phosphorylation were indirect since they were blocked either by the antibodies to IL-4 and IL-13 or in IL-4 knock out mice in the presence of IL-13 antibody. These observations suggest that histamine indirectly affected the STAT6 phosphorylation via its effects on the secretion of cytokines (IL-4) and H1 receptor played a role in this process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 7, Issue 3, March 2007, Pages 277-286
Journal: International Immunopharmacology - Volume 7, Issue 3, March 2007, Pages 277-286
نویسندگان
Geetanjali Kharmate, Zhongfeng Liu, Eric Patterson, Manzoor M. Khan,