کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2546737 | 1124034 | 2009 | 5 صفحه PDF | دانلود رایگان |
Aim of the StudyThere is greater consumer awareness of plant-based skin-care products. Sophora japonica L. (Fabaceae) has been used traditionally as a hemostatic agent and also has skin-care and whitening benefits. The effect of the isolated active compounds of Sophora japonica L. (Fabaceae) that inhibits tyrosinase activity in human epidermal melanocytes (HEMn) was examined.Materials and methodsWe used the mushroom tyrosinase inhibitory assay to isolate active constituents from the extracts. The structures of these constituents were characterized by physical and spectroscopic analyses. Cellular tyrosinase kinetics were analyzed and showed by Lineweaver–Burk plot.ResultsA new compound, N-feruloyl-N′-cis-feruloyl-putrescine (8), together with four flavonoids and three putrescine derivatives were obtained after assay-guided isolation of S. japonica. In HEMn, compound 8 was minimally cytotoxic (cell viability >90% at 100 μM) and the IC50 value for suppression of cellular tyrosinase activity was estimated as 85.0 μM. Zymography analysis demonstrated the compound's concentration-dependent effects and the kinetic analysis indicated the compound's mixed-inhibitory action.ConclusionsWe concluded that the new compound 8 is the most potent component of S. japonica yet discovered. Its pigment inhibition activity may be exploitable cosmetically.
A new compound, N-feruloyl-N′-cis-feruloyl-putrescine (8), together with four flavonoids and three putrescine derivatives were obtained from Sophora japonica L. (Fabaceae). Compound 8 was minimally cytotoxic (cell viability >90% at 100 μM) and the IC50 value for suppression of cellular tyrosinase activity was estimated as 85.0 μM in human epidermal melanocytes (HEMn). Its pigment inhibition activity may be candidates for cosmetic use.Figure optionsDownload as PowerPoint slide
Journal: Journal of Ethnopharmacology - Volume 124, Issue 3, 30 July 2009, Pages 625–629