کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2550624 1560584 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway
چکیده انگلیسی

AimsThis study aims to evaluate the renoprotective effect of betulin and the possible mechanisms in septic rats.MethodsIn the animal study, rats underwent cecal ligation and puncture (CLP) were used as septic models. Betulin 4 mg/kg or 8 mg/kg was administrated intraperitoneally immediately after the model establishment. In the cell study, lipopolysaccharide (LPS) stimulated rat HBZY-1 mesangial cells were used. Cells were pretreated with betulin before the LPS stimulation. Histological change of kidney was examined using periodic acid-schiff staining. Serum creatinine and blood urea nitrogen were detected using biochemical assay kits, and proinflammatory factors in kidney and culture medium were detected using ELISA kits. Immunohistochemistry analysis was used to measure F4/80, HMGB-1 and TLR4 expression in renal tissue of rats. mRNA and protein expressions of HMGB-1, TLR4, IκBα, p65 and p-p65 were measured using Real-time PCR and Western blot, respectively. Nuclear factor kappa-B (NF-κB) nuclear translocation was observed using immunofluorescence staining.Key findingsResults showed that betulin attenuated CLP-induced renal damage, reduced levels of serum creatinine and blood urea nitrogen, and decreased the proinflammatory cytokines secretion in the kidney of septic rats and culture medium of LPS-stimulated cells. Betulin could also downregulated HMGB-1 and TLR4 mRNA and protein expression in the kidney of septic rats, as well as inhibited NF-κB signal activation.SignificanceOur finding suggests that betulin attenuates kidney injury in septic rats through anti-inflammation and TLR4/NF-κB signaling inhibition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 144, 1 January 2016, Pages 185–193
نویسندگان
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