کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2550896 1560594 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Curcumin inhibits Ec109 cell growth via an AMPK-mediated metabolic switch
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Curcumin inhibits Ec109 cell growth via an AMPK-mediated metabolic switch
چکیده انگلیسی

AimsGlycolytic enzymes are always greatly increased in cancer cells. Whether metabolic reprogramming is involved in curcumin-mediated inhibition of cancer cell growth is unknown.Main methodsIn this study, cell viability was assayed with MTS analysis; cell cycle was measured with flow cytometry analysis. RT-PCR and western blotting were used to analyse the mRNA and protein expression, respectively.Key findingsHere we demonstrated that curcumin inhibited cancer cell growth, especially for Ec109 cells. Curcumin induced cell cycle arrest at G2/M phase. Curcumin caused a significant down-regulation of glycolytic enzymes expressions in a dose-dependent manner. Our results further indicated that the AMPK was required for curcumin-mediated down-regulation of glycolytic enzymes. AMPK-mediated down-regulation of glycolytic enzymes blocked Ec109 cell growth.SignificanceTaken together, our results revealed that the AMPK-mediated metabolic switch plays an important role in esophageal cancer cell growth.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 134, 1 August 2015, Pages 49–55
نویسندگان
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