کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2552448 | 1560688 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Glucose stimulation of protein acylation in the pancreatic β-cell Glucose stimulation of protein acylation in the pancreatic β-cell](/preview/png/2552448.png)
AimsTo determine whether protein acylation plays a role in the effects of glucose on the insulin secreting β-cell.Main methodsThe measurement of 3H-palmitate incorporation into protein in the INS 832/13 cell that has a robust and well-characterized biphasic insulin secretory response to stimulation with glucose.Key findingsStimulating the cells with glucose increased the incorporation of 3H-palmitic acid into protein by up to 90%. Similarly, 2-aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) the non-metabolizable analog of leucine that mimics the stimulatory effect of glucose on insulin secretion also increased the incorporation of 3H-palmitic acid into protein. Treatment of cell lysates with hydroxylamine substantially reduced the incorporation indicating that most of the incorporation was due to enzymatic palmitoylation of proteins. Cerulenin, a classical inhibitor of protein acylation also substantially reduced the incorporation. Using PAGE and autoradiography a glucose-induced increase in protein palmitoylation and specific glucose-induced increases in the palmitoylation of proteins of 30, 44, 48 and 76 kD were identified.SignificanceThe data suggest that protein acylation plays multiple roles in β-cell function.
Journal: Life Sciences - Volume 87, Issues 23–26, 18 December 2010, Pages 667–671