کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2552728 | 1560757 | 2008 | 7 صفحه PDF | دانلود رایگان |
Postconditioning can induce cardioprotection against ischemia. However, the data on postconditioning of the spinal cord is very limited. We investigated here whether co-application of ischemic preconditioning (IPC) and postconditioning can provide additive neuroprotection against prolonged spinal cord ischemia. Spinal cord ischemia was produced in rabbits by infrarenal aortic occlusion with a balloon catheter for 30 min. The four treatment groups were control (n = 10): no intervention; IPC (n = 10): a 5-minute aortic occlusion was performed 20 min before the prolonged ischemia; Postcon (n = 10): postconditioning comprised of four cycles of 1-minute occlusion/1-minute reperfusion was applied one minute after the start of reperfusion. IPC + postcon (n = 11): both IPC and postconditioning were applied. Functional evaluation with Tarlov score was performed during a 14-day observation period. Neurologic impairment was noticeably attenuated in the IPC + postcon group (compared with the control group, P < 0.01, at day 1, day 2, day 7 and day 14, respectively), but not in either the IPC or Postcon group. Plasma malondialdehyde levels after reperfusion were significantly decreased to a similar extent in the IPC, Postcon and IPC + Postcon groups (compared with the control group (P < 0.01). In the IPC + Postcon group, many more large motor neurons were preserved than in the control group (P < 0.05) and white matter injury was also markedly attenuated as evidenced by reduction of the vacuolation area of the white matter (P < 0.01) and decreased amyloid precursor protein immunoreactivity (P < 0.01). From this, we conclude that the combination of IPC and postconditioning induces additive neuroprotective effects for spinal cord against ischemia and reperfusion injuries.
Journal: Life Sciences - Volume 82, Issues 11–12, 12 March 2008, Pages 608–614