Perinatal exposure of rats to Bisphenol A affects the fertility of male offspring

AimsThe exposure to endocrine disruptor (ED) induces functional and behavioral abnormalities associated with reproduction. Humans are ubiquitously exposed to Bisphenol A (BPA), an ED, as it leaches from polycarbonate plastics into their contents. The aim of the present study was to determine the effect of perinatal exposure of male rats to BPA on fertility parameters and perturbations in the expression of testicular steroid receptors (SRs) in adult F1 offspring. These effects were studied in adult males of the F2 and F3 generations to determine the vertical transmission of BPA exposure.Main methodsPregnant female rats (F0) were gavaged with either BPA (1.2 and 2.4 μg/kg bw), a vehicle control or positive control with Diethylstilbestrol (10 μg/kg bw) during the perinatal period. Adult F1 males were subjected to fertility assessment by mating with unexposed females. The reproductive functions of the subsequent F2 and F3 litters were investigated in a similar manner. Immunohistochemical localization of SRs was carried out in the testes of F1, F2 and F3 generation adult rats.Key findingsA significant increase in post implantation loss and a decrease in litter size and sperm count and motility were observed in the F1 male offspring. A reduction in the testicular expression profile of SRs was observed. These effects were very prominent in the subsequent F2 and F3 generations.SignificancePerinatal exposure to environmentally relevant doses of BPA affects the male germ line, leading to impairments in the fertility of F1 male offspring and their subsequent F2 and F3 generations.