کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2553635 1124916 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of angiogenic differentiation of human umbilical vein endothelial cells by diallyl disulfide (DADS)
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Inhibition of angiogenic differentiation of human umbilical vein endothelial cells by diallyl disulfide (DADS)
چکیده انگلیسی
Angiogenesis is a crucial step in the growth and metastasis of cancers. The activation of endothelial cells and their further behaviour are very critical during angiogenesis. We analyzed the effect of diallyl disulfide (DADS) on angiogenesis in in vitro models using human umbilical vein endothelial cells (HUVECs). DADS significantly inhibited endothelial cell migration, invasion and tube formation. 3H-thymidine proliferation assay clearly showed the inhibitory effect of DADS on the proliferation of HUVECs in vitro. The role of metalloproteinases has been shown to be important in angiogenesis; therefore, zymography was performed to determine whether DADS affected protease activity. Gelatin zymographic analysis showed the inhibitory effect of DADS on the activation of matrix metalloproteinases-MMP-2 and MMP-9. These findings suggest that DADS acts as an angiogenesis inhibitor by inhibiting the activation of matrix metalloproteinases during endothelial morphogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 80, Issue 6, 16 January 2007, Pages 515-521
نویسندگان
, ,