کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2554633 1124980 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vascular and renal effects of dopamine during extracellular volume expansion: Role of nitric oxide pathway
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Vascular and renal effects of dopamine during extracellular volume expansion: Role of nitric oxide pathway
چکیده انگلیسی

ObjectiveThe aim of the study was to determine the possible role of NO-system activation in vascular and renal effects of the dopaminergic system and the probable interaction between both systems during acute volume expansion in rats.Design and methodsExpanded (10% bw) and non-expanded anaesthetized male Wistar rats were treated with haloperidol, a DA receptor antagonist (3 mg/kg bw, ip). Mean arterial pressure, diuresis, natriuresis, renal plasma flow, glomerular filtration rate, nitrites and nitrates excretion (NOx) were determined. NADPH diaphorase activity was measured using a histochemistry technique in kidney, aorta and renal arteries. NOS activity in kidney and aorta from expanded and non-expanded animals was determined with l-[U14C]-arginine substrate, in basal conditions and after DA (1 μM) administration.ResultsThe hypotensive effect of l-arg and hypertension induced by l-NAME were not modified by haloperidol. This blocker reverted the increase in diuresis, natriuresis and RPF induced by l-arg in both groups. Dopaminergic blockade induced a decrease in NOx excretion and in NADPH-diaphorase activity in glomeruli, proximal tubule and medullar collecting duct and in endothelium and vascular smooth muscle of renal arteries. DA induced an increase in NOS activity in renal medulla and cortex in both groups, but no changes in the aorta were observed.ConclusionsOur results suggest that renal DA would be associated with the renal response induced by NO during extracellular volume expansion. NO-system activation would be one of the mechanisms involved in renal DA activity during saline load, but NO appears not to be involved in DA vascular effects.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 78, Issue 14, 28 February 2006, Pages 1543–1549
نویسندگان
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