کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2554806 1124992 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ceramide-induced preconditioning involves reactive oxygen species
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Ceramide-induced preconditioning involves reactive oxygen species
چکیده انگلیسی

IntroductionCeramide induces programmed cell death and it is thought to contribute to cardiac ischemia/reperfusion (I/R) injury. In contrast, we have demonstrated that administration of low doses of ceramide engenders cardiac preconditioning (PC). Ceramide is known to generate reactive oxygen species (ROS) in cells. Since mechanisms triggering the ceramide-induced cardioprotection remain unknown, we investigated the role of ROS in the genesis of this protective mechanism.MethodsUsing an isolated Langendorff-perfused rat heart model, four groups (n ≥ 6 in each group) were considered: Control hearts underwent 30 min index regional ischemia and 120 min of reperfusion. In the ceramide group, hearts were preconditioned with c2-ceramide 1 μM for 7 min followed by 10 min washout prior to the I/R insult. In additional groups, MPG (1 mM), a synthetic antioxidant was given for 15 min alone or bracketing the ceramide perfusion. In each group, infarct size was determined at the end of the reperfusion period and superoxide dismutases (CuZnSOD and MnSOD) and catalase activities were evaluated.ResultsCeramide preconditioning reduced the infarct/area at risk (I/AAR) ratio (8.3 ± 1.1% for ceramide vs. 36.4 ± 1.2% for control, p < 0.001). Perfusion with MPG abolished the preconditioning effect of ceramide (I/AAR ratio = 36.7 ± 4.9%). Ceramide was also associated with a 29% and 38% increase in catalase and CuZnSOD activities, respectively, compared with control group.ConclusionProduction of reactive oxygen species following ceramide preconditioning of the ischemic–reperfused heart appears to play a role in the cardioprotective effect of ceramide.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 78, Issue 15, 6 March 2006, Pages 1702–1706
نویسندگان
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