کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2554873 1124999 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of intracellular delivery of energy metabolites on intracellular Ca2+ in mouse islets of Langerhans
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Effect of intracellular delivery of energy metabolites on intracellular Ca2+ in mouse islets of Langerhans
چکیده انگلیسی
Regulation of glucose-induced oscillations in intracellular Ca2+ concentration ([Ca2+]i) was investigated by using a novel technique, electroporation from an electrolyte-filled capillary, to deliver energy metabolites to the intracellular compartment of mouse islets. Intracellular application of ATP resulted in a nifedipine-sensitive increase in [Ca2+]i, consistent with a KATP-channel dependent mechanism of Ca2+ influx. [Ca2+]i in islets exposed to 10 mM glucose oscillated with a period of ∼3 min, often superimposed with faster oscillations. Electroporation of ATP blocked all types of oscillations and elevated [Ca2+]i while delivery of ADP had no effect on oscillations. Intracellular delivery of glucose-6-phosphate or fructose-1,6-bisphosphate tended to transform slow oscillations to fast oscillations. These results demonstrate that modulation of ATP concentrations and glycolytic flux are important in development of slow oscillations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 77, Issue 23, 21 October 2005, Pages 2986-2997
نویسندگان
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