کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2555038 | 1125022 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cholinesterase activity in human pulmonary arteries and veins: correlation with mRNA levels
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
کاردیولوژی و پزشکی قلب و عروق
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![عکس صفحه اول مقاله: Cholinesterase activity in human pulmonary arteries and veins: correlation with mRNA levels Cholinesterase activity in human pulmonary arteries and veins: correlation with mRNA levels](/preview/png/2555038.png)
چکیده انگلیسی
Isolated intact human pulmonary arteries and veins were used to determine the acetylcholinesterase (AChE) or butyrylcholinesterase (BChE) activities in the absence or presence of two selective cholinesterase (ChE) inhibitors, iso-OMPA or BW284c51, respectively. These results were compared with the mRNA levels for each enzyme in human pulmonary vessels. Total ChE activities measured in presence of acetylthiocholine (ACTI, 1 mM) in intact vascular preparations were 45 ± 04 and 114 ± 07 mU/g tissue in human pulmonary arteries (n = 14) and veins (n = 14), respectively. These activities were completely abolished in presence of 10 μM neostigmine. In both types of vessels AChE and BChE activities were observed. These activities were at least 2-fold higher in human pulmonary veins when compared with arteries and were correlated with the accumulation of the corresponding transcripts (n = 8). In each type of vessel, similar total ChE activities were detected in homogenized and intact preparations, while in human bronchial preparations this activity was 5-fold higher in homogenates than in intact preparations. Together these results provide evidence that the ChE activities in human pulmonary vessels may be extracellular and that the higher activity measured in veins as compared to arteries was associated with the differential accumulation of the corresponding transcripts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 76, Issue 19, 25 March 2005, Pages 2211-2220
Journal: Life Sciences - Volume 76, Issue 19, 25 March 2005, Pages 2211-2220
نویسندگان
Larissa Kotelevets, Laurence Walch, Eric Chastre, Arnaud Chatonnet, Elisabeth Dulmet, Charles Brink, Xavier Norel,