کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
25566 | 43581 | 2006 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Production of exopolysaccharides by submerged mycelial culture of a mushroom Tremella fuciformis Production of exopolysaccharides by submerged mycelial culture of a mushroom Tremella fuciformis](/preview/png/25566.png)
The optimization of submerged culture conditions for mycelial growth and exopolysaccharide (EPS) production in an edible mushroom Tremella fuciformis was studied in shake flasks and bioreactors. The temperature of 28 °C and pH 8 in the beginning of fermentation in agitated flasks was the most efficient condition to obtain maximum mycelial biomass and EPS. The optimal medium constituents were as follows (g L−1): glucose 20, tryptone 2, KH2PO4 0.46, K2HPO4 1 and MgSO4·7H2O 0.5. The fungus was cultivated under various agitation and aeration conditions in a 5 L stirred-tank bioreactor. The maximum cell mass and EPS production were obtained at a relatively high agitation speed of 200 rpm and at an aeration rate of 2 vvm. The flow behavior of the fermentation broth was Newtonian and the maximum apparent viscosity (35 cP) was observed at a highly aerated condition (2 vvm). The EPS productivity in an airlift reactor was higher than that in the stirred-tank reactor. The morphological study revealed that the fungus grows in mainly three different yeast-like forms: ovoid, elongated, and double yeast forms. The high population of the elongated yeast has a very close relationship to high EPS production. The EPS were protein-bound polysaccharides consisted of mainly mannose, xylose, and fucose. The molecular weights of EPS were determined to be (1.3–1.5) × 106.
Journal: Journal of Biotechnology - Volume 127, Issue 1, 15 December 2006, Pages 129–140