کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
25673 | 43589 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Regioselectivity of 7-O-methyltransferase of poplar to flavones Regioselectivity of 7-O-methyltransferase of poplar to flavones](/preview/png/25673.png)
POMT-7, an O-methyltransferase from poplar (Populus deltoids) was used to modify a variety of flavonoid compounds. POMT-7 was able to transfer a methyl group to several flavonoids containing a C-7 hydroxyl group. However, POMT-7 showed a higher affinity toward flavonol and flavone such as apigenin, kaempferol, luteolin, and quercetin than flavanone and isoflavone. Based on comparison of HPLC retention times with authentic compounds and corresponding nuclear magnetic resonance spectroscopy data, the methylation position of the reaction products was determined to be at the hydroxyl group of C-7. Biotransformation kinetics indicated that the enzyme converted more than 80% of the apigenin, kaempferol, luteolin and quercetin substrates, which were added at concentration of 70 μM, into corresponding 7-methoxy compounds within 24 h.
Journal: Journal of Biotechnology - Volume 126, Issue 2, 1 November 2006, Pages 241–247