کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2568296 1128432 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolic activation of hepatotoxic drug (benzbromarone) induced mitochondrial membrane permeability transition
ترجمه فارسی عنوان
فعال سازی متابولیک از داروهای هپاتوتوکسی (بنزبروارن) باعث انتقال نفوذپذیری غشای میتوکندری
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


• We constructed a sequential assay system for toxic metabolite induced MPT in one pot.
• 14 drugs (e.g. benzbromarone (BBR)) induced toxic metabolite dependent MPT.
• Both the production of toxic metabolite and MPT could be inhibited by CYP inhibitors.
• This system could evaluate the comprehensive MPT without purification of metabolites.

The risk of drug-induced liver injury (DILI) is of great concern to the pharmaceutical industry. It is well-known that metabolic activation of drugs to form toxic metabolites (TMs) is strongly associated with DILI onset. Drug-induced mitochondrial dysfunction is also strongly associated with increased risk of DILI. However, it is difficult to determine the target of TMs associated with exacerbation of DILI because of difficulties in identifying and purifying TMs. In this study, we propose a sequential in vitro assay system to assess TM formation and their ability to induce mitochondrial permeability transition (MPT) in a one-pot process. In this assay system, freshly-isolated rat liver mitochondria were incubated with reaction solutions of 44 test drugs preincubated with liver microsomes in the presence or absence of NADPH; then, NADPH-dependent MPT pore opening was assessed as mitochondrial swelling. In this assay system, several hepatotoxic drugs, including benzbromarone (BBR), significantly induced MPT in a NADPH-dependent manner. We investigated the rationality of using BBR as a model drug, since it showed the most prominent MPT in our assay system. Both the production of a candidate toxic metabolite of BBR (1′,6-(OH)2 BBR) and NADPH-dependent MPT were inhibited by several cytochrome P450 (CYP) inhibitors (clotrimazole and SKF-525A, 100 μM). In summary, this assay system can be used to evaluate comprehensive metabolite-dependent MPT without identification or purification of metabolites.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 288, Issue 1, 1 October 2015, Pages 12–18
نویسندگان
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