کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2574782 1561276 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
IL-18 accelerates the cell apoptosis by up-regulating Cysteinyl Leukotriene 2 Receptor Expression in Human Umbilical Vein Endothelial Cells at the early stage of administration
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
IL-18 accelerates the cell apoptosis by up-regulating Cysteinyl Leukotriene 2 Receptor Expression in Human Umbilical Vein Endothelial Cells at the early stage of administration
چکیده انگلیسی

The purpose of the present study is to identify whether interleukin (IL)-18 can modulate cysteinyl leukotriene 2 receptor (CysLT2R) expression in Human Umbilical Vein Endothelial Cells (HUVECs) and how it influences the cell death. According to the results from real-time reverse transcription PCR, confocal laser scanning microscopy, and western blotting, a dose-dependent augmentation of CysLT2R protein expression in HUVECs was triggered by IL-18 for the first 2 h followed by down-regulation within the next 22 h after IL-18 administration. The flow cytometry showed that non-selective CysLT1R and CysLT2R antagonist BAY-u9773 could attenuate the early stage apoptosis mediated by IL-18 whereas CysLT1R antagonist Montelukast couldn't. Also, pretreatment with BAY-u9773 suppressed calcium influx of HUVECs induced by IL-18 whereas Montelukast didn't work. The observation that progression of cell death aggravated by IL-18 could be attenuated by BAY-u9773 may offer a chance to develop a novel way to treat arteriosclerosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vascular Pharmacology - Volume 50, Issues 5–6, May–June 2009, Pages 171–177
نویسندگان
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