کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2582892 1561703 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Grafting of gallic acid onto chitosan nano particles enhances antioxidant activities in vitro and protects against ochratoxin A toxicity in catfish (Clarias gariepinus)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Grafting of gallic acid onto chitosan nano particles enhances antioxidant activities in vitro and protects against ochratoxin A toxicity in catfish (Clarias gariepinus)
چکیده انگلیسی


• Ochratoxin-A (OTA) is a mycotoxin produced by Penicillium and Asperigillus.
• OTA is hepatonephrotoxic, teratogenic and immunosuppressive agent.
• Applicability of chitosan (CS) is limited due to its poor solubility.
• CS nanoparticles (CSNPs) was modified and grafted with gallic acid or octyl gallate.
• Modified CSNPs has higher antioxidant activity in vitro & protect against OTA in vivo.

This study aimed to prepare and characterize enzymatic modified chitosan nanoparticles (CSNPs) with gallic acid (GA) or octyl gallate (OG) to optimize its potential in human application and to evaluate their protective role against ochrtoxin A (OTA) toxicity in catfish. The modified CSNPs have average size around 90 nm with positive charge and high scavenging activity especially GA-CSNPs. In the in vivo study, catfish were divided into 8 groups and treated for 3 weeks as follow: the control group, OTA-treated group (1 mg/kg b.w.), the groups treated with CSNPs, GA-CSNPs or OG-CSNPs (280 mg/kg b.w.) anole or in combination with OTA. Blood, liver and kidney samples were collected for different analyses. OTA induced a significant biochemical disturbances accompanied with oxidative stress in liver and kidney, histological changes and increase DNA fragmentation in the kidney. Co-treatment with OTA plus the different CSNPs resulted in a significant improvement in all tested parameters and histological picture of the kidney. This improvement was more pronounced in the group treated with GA-CSNPs. It could be concluded that grafting of GA or its ester improved the properties of CSNPs. Moreover, GA-CSNPs showed strong scavenging properties than OG-CSNPs due to the blocking of carboxyl groups responsible of the scavenging activity in OG.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 41, January 2016, Pages 279–288
نویسندگان
, , , , , ,