کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2590851 1562081 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of colistin on amino acid neurotransmitters and blood-brain barrier in the mouse brain
ترجمه فارسی عنوان
اثر کلستین بر روی انتقال دهنده های عصبی آمینو اسید و مانع خون مغزی در مغز موش
کلمات کلیدی
BBB، مانع خونریزی مغزی EB، ایوانز آبی؛ TEM، میکروسکوپ الکترونی انتقال؛ HPLC، کروماتوگرافی مایع با کارایی بالا؛ گلو، گلوتامات؛ GABA، گاما آمینو بوتیریک اسید؛ GABAAR، گیرنده گاما آمینو بوتیریک اسید نوع A؛ GABABR، اسید گاما آمینو بوتیریک
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


• Colistin exposure did not affect the integrity of blood brain barrier in mouse.
• Repeated colistin exposure can cause accumulation of colistin in mouse brain.
• Colistin caused significant increase of the amount of Glu and GABA, and also effect the receptors in mouse cortex.

Neurotoxicity is one of the major potential side effects of colistin therapy. However, the mechanistic aspects of colistin-induced neurotoxicity remain largely unknown. The objective of this study was to examine the effects of colistin on the blood-brain barrier (BBB) and amino acid neurotransmitters in the cerebral cortex of mouse. Mice were divided into four groups (n = 5) and were administrated intravenously with 15 mg/kg/day of colistin sulfate for 1, 3 and 7 days successively while the control group was administrated intravenously with saline solution. The permeability and ultrastructure of the BBB were detected using the Evans blue (EB) dye and transmission electron microscopy (TEM), and the expression of Claudin-5 were determined by real-time PCR examination and western blotting. The brain uptake of colistin was measured by high-performance liquid chromatography (HPLC). The effects of colistin on amino acid neurotransmitters and their receptors were also examined by HPLC and real-time PCR. The results of EB extravasation, TEM and expression of Claudin-5 showed that colistin treatment did not affect the BBB integrity. In addition, multiple doses of colistin could induce accumulation of this compound in the brain parenchyma although there was poor brain uptake of colistin. Moreover, colistin exposure significantly increased the contents of glutamate (Glu) and gamma aminobutyric acid (GABA), and enhanced the mRNA expression levels of gamma aminobutyric acid type A receptor (GABAAR), gamma aminobutyric acid type B receptor (GABABR), N-methyl-d-aspartate 1 receptor (NR1), N-methyl-d-aspartate 2A receptor (NR2A) and N-methyl-d-aspartate 2B receptor (NR2B) in the cerebral cortex. Our data demonstrate that colistin is able to accumulate in the mouse brain and elevate the levels of amino acid neurotransmitters. These findings may be associated with colistin-induced neurotoxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurotoxicology and Teratology - Volume 55, May–June 2016, Pages 32–37
نویسندگان
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