کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2593849 1132251 2012 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Zebrafish developmental screening of the ToxCast™ Phase I chemical library
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Zebrafish developmental screening of the ToxCast™ Phase I chemical library
چکیده انگلیسی

Zebrafish (Danio rerio) is an emerging toxicity screening model for both human health and ecology. As part of the Computational Toxicology Research Program of the U.S. EPA, the toxicity of the 309 ToxCast™ Phase I chemicals was assessed using a zebrafish screen for developmental toxicity. All exposures were by immersion from 6–8 h post fertilization (hpf) to 5 days post fertilization (dpf); nominal concentration range of 1 nM–80 μM. On 6 dpf larvae were assessed for death and overt structural defects. Results revealed that the majority (62%) of chemicals were toxic to the developing zebrafish; both toxicity incidence and potency was correlated with chemical class and hydrophobicity (logP); and inter-and intra-plate replicates showed good agreement. The zebrafish embryo screen, by providing an integrated model of the developing vertebrate, compliments the ToxCast assay portfolio and has the potential to provide information relative to overt and organismal toxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 33, Issue 2, April 2012, Pages 174–187
نویسندگان
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